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Determination of glucose by capillary electrophoresis/laser‐induced fluorescence in transdermally collected samples
Author(s) -
Frerichs Valerie,
Colón Luis A.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191510
Subject(s) - capillary electrophoresis , transdermal , chromatography , fluorescence , diffusion , chemistry , laser induced fluorescence , capillary action , analytical chemistry (journal) , electrophoresis , laser , materials science , optics , medicine , physics , composite material , pharmacology , thermodynamics
Capillary electrophoresis (CE) with laser‐induced fluorescence (LIF) detection has been used for the determination of glucose in samples collected by noninvasive means. The method uses an enzymatic reaction scheme that provides for the determination of small quantities of glucose with detection limits of 80 n M . This approach is used to evaluate passive transdermal diffusion as a noninvasive means to sample glucose in vivo. A simple sampling cell design is presented. Sample collection was performed on volunteer human subjects. Our experiments show that fluctuations in blood glucose concentration are reflected in the samples obtained by passive transdermal diffusion after glucose intake. The results indicate that glucose from the subcutaneous fluid can be accessed by passive diffusion.