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Differential expression of cytosolic proteins in the rat kidney cortex and medulla: Preliminary proteomics
Author(s) -
Witzmann Frank A.,
Fultz Carla D.,
Grant Raymond A.,
Wright Lynda S.,
Kornguth Steven E.,
Siegel Frank L.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191423
Subject(s) - cortex (anatomy) , medulla , proteome , proteomics , kidney , biology , renal cortex , chemistry , mass spectrometry , microbiology and biotechnology , biochemistry , anatomy , chromatography , endocrinology , neuroscience , gene
Abstract The rodent kidney is a target of many xenobiotics and is typified by regionally specific structure and function. This renders distinct regions of the kidney differentially susceptible to toxic exposure and effect. To characterize these differences at the proteome level, protein patterns from male rat kidney cortex and medulla cytosols were examined by two‐dimensional electrophoresis (2‐DE) and image analysis and prominent proteins identified immunologically or by matrix‐assisted laser desorption/ionization ‐ mass spectrometry (MALDI‐MS) and electrospray/ionization ‐ tandem mass spectrometry (ESI‐MS/MS) sequence tag identification. An average of 727 protein spots were resolved and matched to the cortex cytosol reference pattern, and 716 in the medulla. Of this total, 127 proteins were found to differ in abundance (86 higher in cortex; 41 higher in medulla) ( P < 0.001). Of those proteins that were detectable in both cortex and medulla, the abundance of 97 differed significantly while 30 proteins were found to be unique to one region or the other (26 in cortex, 4 in medulla). Twenty protein spots were identified and their regional differences are discussed. These results both confirm and expand our understanding of the molecular heterogeneity characterizing structurally and functionally distinct regions of the kidney and serve as a useful foundation for future nephrotoxicologic studies.