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Separation of inorganic and organic anions by capillary zone electrophoresis with simultaneous indirect UV and conductivity detection
Author(s) -
Klampfl Christian W.,
Katzmayr Martin U.,
Buchberger Wolfgang
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191418
Subject(s) - capillary electrophoresis , chemistry , electrolyte , conductivity , tris , electrophoresis , hydroxymethyl , chromatography , analyte , molar absorptivity , organic anion , analytical chemistry (journal) , theoretical plate , ion , organic chemistry , electrode , biochemistry , physics , optics
The analysis of low‐molecular‐mass anionic compounds using capillary zone electrophoresis (CZE) with simultaneous direct conductivity and indirect UV detection is described. A number of carrier electrolyte compositions has been investigated with respect to the crucial parameters for their compatibility with both detection principles discussed in this paper (UV absorptivity and electrophoretic mobility of the buffer coion). Additionally, parameters including pH and buffer concentration affecting both peak symmetry and signal‐to‐noise ratio obtained for the analytes of interest had to be optimized. Best results could be achieved with a carrier electrolyte consisting of 4‐aminobenzoic acid, bis(2‐hydroxyethyl)imino‐tris(hydroxymethyl)aminomethane (BIS‐Tris) and a pH of 7.0 adjusted with LiOH. This running buffer was used for the analysis of low‐molecular‐mass anionic compounds in a sample of white wine.