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Mixed‐dye staining method for protein detection in polyacrylamide gel electrophoresis using calconcarboxylic acid and rhodamine B
Author(s) -
Jung DaWoon,
Yoo GyurngSoo,
Choi JungKap
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191409
Subject(s) - gel electrophoresis of proteins , staining , chromatography , polyacrylamide gel electrophoresis , rhodamine b , color marker , chemistry , electrophoresis , rhodamine , gel electrophoresis , polyacrylamide , two dimensional gel electrophoresis , pulsed field gel electrophoresis , microbiology and biotechnology , biochemistry , fluorescence , biology , proteomics , polymer chemistry , physics , photocatalysis , quantum mechanics , genetics , catalysis , gene , genotype , enzyme
We have developed a new mixed‐dye protein staining method that is simple, rapid, and sensitive. A freshly prepared mixture of calconcarboxylic acid (NN, 0.02%) and rhodamine B (RB, 0.04%) in 40% methanol / 7% acetic acid, was used as a staining solution. RB acts as an auxiliary agent to inhibit the binding of NN to the gel matrix, reducing the background staining and therefore enhancing the protein staining by NN. This mixed‐dye staining method reduces the total staining and destaining time to less than an hour, and increases the sensitivity to 25 ng of bovine serum albumin, which is greater than the 100 ng sensitivity limit of Coomassie Brilliant Blue R‐250 (CBBR) staining.