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Triple helix‐forming oligonucleotides which make imperfect Watson‐Crick duplexes that compete with the creation of the triplex
Author(s) -
Porumb Horea,
Gousset Hervé,
Taillandier Eliane
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191404
Subject(s) - triple helix , duplex (building) , oligonucleotide , molecular structure of nucleic acids: a structure for deoxyribose nucleic acid , helix (gastropod) , pyrimidine , chemistry , stereochemistry , biology , base pair , dna , biochemistry , ecology , snail
Because of the repetitive nature of the sequence, when titrating a G,A‐rich, triple helix‐forming oligonucleotide (TFO) with increasing concentrations of target duplex in order to obtain the dissociation constant of the complex, a duplex is sometimes first generated at intermediate concentrations of the target. This duplex is based on an imperfect Watson‐Crick pairing of the TFO to the pyrimidine‐rich strand of the target. An explanation is proposed for this duplex being obtained only in a certain domain of the titration range, before the triple helix becomes predominant.