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Changes in outer membrane protein profiles of bacteria after meropenem‐induced postantibiotic effect studied by capillary electrophoresis
Author(s) -
Kustos Ildikó,
Kocsis Béla,
Kerepesi Ildikó,
Kilár Ferenc
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191312
Subject(s) - meropenem , capillary electrophoresis , bacteria , bacterial outer membrane , sodium dodecyl sulfate , gel electrophoresis , polyacrylamide gel electrophoresis , electrophoresis , microbiology and biotechnology , chemistry , membrane protein , enterobacteriaceae , chromatography , biology , membrane , biochemistry , escherichia coli , enzyme , gene , antibiotic resistance , genetics
Persistent inhibition of bacterial growth, called postantibiotic effect (PAE), after a short exposure to a new carbapenem, meropenem, was determined in different strains of the Enterobacteriaceae family. Capillary electrophoresis (CE), as well as sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) were used to study the outer membrane protein (OMP) profiles before and after meropenem treatment. CE proved to be suitable for the characterization of the OMP profiles of bacteria. Significant changes in the electrophoretic patterns were observed, showing the consequential effect of meropenem on bacteria.