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Protein profile characterization of bacterial lysates by capillary electrophoresis
Author(s) -
Kustos Ildikó,
Kocsis Béla,
Kerepesi Ildikó,
Kilár Ferenc
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191311
Subject(s) - electropherogram , capillary electrophoresis , chromatography , electrophoresis , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , gel electrophoresis , chemistry , biochemistry , enzyme
A fast and reproducible method was developed to characterize cell lysates by their electrophoretic profiles using capillary electrophoresis (CE). Characteristic and reproducible patterns were recorded for each bacterial strains when “dynamic sieving” CE, using a polymer solution in the capillary, was applied to distinguish four strains of the Enterobacteriaceae family. The electropherograms showed distinct differences when comparing them to the sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) protein profiles. This is certainly a result of the differences in the separation principles and in the detection methods of the two techniques.