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Recent developments in capillary isoelectric focusing with whole‐column imaging detection
Author(s) -
Fang Xiaohong,
Tragas Charalambos,
Mao Qinglu,
Pawliszyn Janusz,
Wu Jiaqi
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191307
Subject(s) - isoelectric focusing , detector , resolution (logic) , capillary action , chemistry , interference (communication) , chromatography , analytical chemistry (journal) , optics , materials science , computer science , artificial intelligence , physics , computer network , biochemistry , channel (broadcasting) , composite material , enzyme
Capillary isoelectric focusing (CIEF) is a high resolution technique for protein separation. The on‐column single point detector requires a mobilization step which lengthens the analysis time and causes an uneven resolution along the separation column. The real time and whole column imaging detection has been developed for performing CIEF without mobilization. Three types of imaging detection systems have been developed: optical absorption, refractive index gradient, and laser induced fluorescence. This technique provides a fast analysis speed (about 6 min) and a good resolution of 0.03 pH unit level. Using the absorption imaging detector, ampholyte‐free IEF in tapered capillary is being demonstrated, which eliminates the interference of the expensive carrier ampholytes for protein detection in UV region. Recent advancements in this imaged CIEF technique as well as its applications are reviewed.