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Cyclodextrins as selectivity enhancers in capillary zone electrophoresis of proteins
Author(s) -
Rathore Anurag S.,
Horváth Csaba
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191306
Subject(s) - chemistry , capillary electrophoresis , chromatography , cyclodextrin , chymotrypsinogen , selectivity , lysozyme , electrophoresis , aqueous solution , biochemistry , organic chemistry , chymotrypsin , enzyme , trypsin , catalysis
The selectivity in the capillary zone electrophoresis (CZE) of a variety of acidic and basic proteins including α‐chymotrypsinogen A, cytochrome c , lysozyme, ribonuclease A, ovalbumin, and β‐lactoglobulins A and B, was altered by adding 6‐monodeoxy‐6‐monoamino‐β‐cyclodextrin or carboxymethylated β‐cyclodextrin to the electrophoretic medium of aqueous 50 m M sodium phosphate, pH 2.5. On the other hand, no significant improvement was obtained in the separation upon addition of heptakis (2,6‐di‐ O ‐methyl)‐β‐cyclodextrin. Whereas protein adsorption on the wall of raw silica capillaries was significant in the absence of cyclodextrin, by addition of β‐cyclodextrin or its derivatives to the background electrolyte, wall adsorption was reduced with concomitant enhancement of the recovery. The results confirm that in various separation techniques, particularly those which employ microcolumns, certain cyclodextrin additives can be useful selectivity enhancers not only in the separation of small sample molecules but also in that of proteins.