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On‐line capillary electrophoresis‐electrospray ionization mass spectrometry using a polymerized anionic surfactant
Author(s) -
Lu Wenzhe,
Shamsi Shahab A.,
McCarley Tracy D.,
Warner Isiah M.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191225
Subject(s) - chromatography , chemistry , capillary electrophoresis , electrospray ionization , capillary electrophoresis–mass spectrometry , mass spectrometry , electrospray , sodium dodecyl sulfate , analytical chemistry (journal)
On‐line capillary electrophoresis ‐ electrospray ionization ‐ mass spectrometry (CE‐ESI‐MS) has been used to determine the tricyclic antidepressant drugs (imipramine, doxepin, and amitriptyline) as well as the β‐adrenergic blocker drugs (propranolol and alprenolol). A CE‐ESI‐MS interface linking a manually operated CE system and a Finnigan MAT‐900 sector mass spectrometer (with an Analytica electrospray ionization source) has been constructed in‐house and employed for this study. Although a water/methanol based capillary zone electrophoresis (CZE) buffer was initially used to determine these analytes, enhanced resolution was obtained by addition of a polymerized surfactant, i.e. , poly‐sodium undecylenic sulfate (poly‐SUS), into the electrokinetic chromatography (EKC) buffer. When a low concentration of this poly‐SUS surfactant was added to a volatile EKC buffer, these structurally similar cationic drugs were EKC separated and on‐line detected by ESI‐MS.