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Nonaqueous capillary zone electrophoresis for separation of free fatty acids with indirect fluorescence detection
Author(s) -
Wang Tianlin,
Wei Hongping,
Li Sam F. Y.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191224
Subject(s) - capillary electrophoresis , chemistry , chromatography , fluorescence , fluorescein , electrolyte , electrophoresis , methanol , detection limit , acetonitrile , capillary action , fatty acid , free flow electrophoresis , biochemistry , organic chemistry , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , enzyme , materials science , physics , electrode , quantum mechanics , composite material
The feasibility of combining nonaqueous capillary zone electrophoresis with indirect fluorescence detection was studied for the efficient separation and sensitive detection of ionizable hydrophobic substances which do not possess practically suitable detection properties. Medium‐ and long‐chain free fatty acids, C 6 C 24 , were selected as test compounds. The results showed that such a wide range of medium‐ and long‐chain free fatty acids could be separated between any two consecutive homologs in one run and be detected at a level of about 0.01–0.02 m M in highly basic methanol/acetonitrile media containing fluorescein as coion of background electrolyte for indirect fluorescence detection.