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Characterization of micelles by capillary electrophoresis
Author(s) -
Schwarz Maria A.,
Raith Klaus,
Neubert Reinhard H. H.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191218
Subject(s) - capillary electrophoresis , characterization (materials science) , micelle , chromatography , capillary action , chemistry , electrophoresis , materials science , nanotechnology , aqueous solution , composite material
A practical approach for the characterization of pure micelles, and binary or ternary mixed micelles by capillary electrophoretic methods is presented. Interest is focused on the determination of mobility and composition of the micelles. The investigations are performed with bile salts, phosphatidylcho‐lines and fatty acids. Pure bile salt micelles are characterized with the help of marking and displacement methods. Binary bile salt/phospholipid and ternary bile salt/phospholipid/fatty acid micelles are analyzed using capillary electrophoresis (CE) techniques with standard and improved UV detection, laser‐induced fluorescence and electrospray ionization ‐ mass spectrometry (ESI‐MS). For both the binary and the ternary systems, only one stable mixed micellar phase is found with a high negative mobility.