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Capillary electrophoresis of anions at high salt concentrations
Author(s) -
Ding Weiliang,
Thornton Michelle J.,
Fritz James S.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191216
Subject(s) - capillary electrophoresis , electrolyte , chemistry , salt (chemistry) , electrophoresis , chromatography , ionic bonding , chloride , sodium , capillary action , joule heating , analytical chemistry (journal) , ion , materials science , electrode , organic chemistry , composite material
It is commonly thought that even a moderately high ionic concentration in the background electrolyte (BGE) would leaad to Joule heating and serious peak distortion. However, we obtained very satisfactory separations of both inorganic and organic anions in electrolyte solutions as high as 5 M sodium chloride using direct photometric detection. Samples containing a 0.5 M concentration of a salt can be analyzed directly by making the BGE concentration of the same salt even higher to obtain electrostacking. The temperature in the center of the capillary was calculated to be 49°C when the current is at its maximum of 280 μA. The effect of various salts on electrophoretic and electroosmotic mobility is discussed. Several examples are given of capillary electrophoresis under high‐salt conditions.