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Chiral separation of polychlorinated biphenyls by micellar electrokinetic chromatography with sodium cholate
Author(s) -
Navazo Antonio Luis Crego,
Marina María Luisa,
Gonzalez María José
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191212
Subject(s) - micellar electrokinetic chromatography , chromatography , chemistry , sodium cholate , electrokinetic phenomena , sodium , electrophoresis , organic chemistry
Micellar electrokinetic chromatography (MEKC) with one kind of bile salt (sodium cholate) was used to separate three chiral polychlorinated biphenyls (PCBs; 84, 95, and 176), each one in its two enantiomers. Sodium cholate was used as chiral surfactant in a 2‐( N ‐cyclohexylamino) ethanesulfonic acid (CHES) buffer under alkaline (pH 10) conditions containing urea (2 M ). The influence of bile salt concentration on the efficiency and the resolution between the two enantiomers of PCBs 84 and 95 was established. The chiral separation of three PCBs was successfully achieved in less than 30 min (approximately 23 min for PCB 176 and approximately 29 min for PCBs 84 and 95).