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Effects of renovascular hypertension on myocardial protein patterns: Analysis by computer‐assisted two‐dimensional gel electrophoresis
Author(s) -
Pleißner KlausPeter,
RegitzZagrosek Vera,
Krüdewagen Bernd,
Trenkner Jörg,
Hocher Berthold,
Fleck Eckart
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191124
Subject(s) - renovascular hypertension , ventricle , medicine , cardiology , blood pressure , diastole , left ventricular hypertrophy , endocrinology
Hypertensive heart disease caused by renovascular hypertension reflects the response of the heart to an increased afterload and neurohormonal stimulation. We hypothesized that in this condition the composition of the myocardial proteins of rats was altered. To identify yet unknown quantitative and qualitative differences in myocardial proteins in renovascular hypertensive heart disease, we analyzed protein patterns by computer‐assisted two‐dimensional polyacrylamide large gel electrophoresis. Renovascular hypertension was induced by placing a silver clip on the left renal artery in 9‐week‐old rat siblings. Sham‐operated animals served as controls. Systolic blood pressure (197 ± 19 mm Hg) and heart/body weight ratios (0.36 ± 0.04%) were significantly increased in the hypertensive animals. Twenty protein patterns from the left ventricle of five hypertensive and five control rats were compared. The molecular mass and isoelectric point (p I ) of proteins spots ranging from 13 to 100 kDa and from 4.5 to 8.5, respectively, were determined using marker proteins. In total, 761 ± 88 protein spots were resolved in all twenty gels. For the quantitative data analysis a univariate (Mann‐Whitney test) as well as a multi‐variate statistical approach (correspondence analysis) were applied. Only one myocardial protein spot (molecular mass = 41.3 kDa; p I = 6.3) was decreased by more than twofold ( p < 0.05) in renovascular hypertension. The vast majority of spots did not indicate a significant alteration of intensity. Left ventricular hypertrophy in early renovascular hypertension induces a form of myocardial hypertrophy that conserves the naturally occurring protein expression pattern.