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New zwitterionic detergents improve the analysis of membrane proteins by two‐dimensional electrophoresis
Author(s) -
Chevallet Mireille,
Santoni Véronique,
Poinas Alexandra,
Rouquié David,
Fuchs Alexandra,
Kieffer Sylvie,
Kieffer Sylvie,
Lunardi Joël,
Garin Jérome,
Rabilloud Thierry
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191108
Subject(s) - chaotropic agent , thiourea , solubility , chromatography , membrane protein , urea , chemistry , electrophoresis , resolution (logic) , membrane , gel electrophoresis , alkyl , biochemistry , organic chemistry , artificial intelligence , computer science
Severe quantitative loss of protein is often observed in high‐resolution two‐dimensional electrophoresis of membrane proteins, while the resolution is usually not affected. To improve the solubility of proteins in this technique, we tested denaturing cocktails containing various detergents and chaotropes. Best results were obtained with a denaturing solution containing urea, thiourea, and zwitterionic detergents, synthesized for this purpose. Among the dozen detergents synthesized and tested, amidosulfobetaines with an alkyl tail containing 14–16 carbons proved most efficient, solubilizing previously undetected membrane proteins.