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Use of a Hepta‐tyr glycopeptide antibiotic as chiral selector in capillary electrophoresis
Author(s) -
Fanali Salvatore,
Aturki Zeineb,
Desiderio Claudia,
Boss Alessandra,
Righetti Pier Giorgio
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191036
Subject(s) - capillary electrophoresis , teicoplanin , chromatography , acetonitrile , chemistry , glycopeptide , electrophoresis , capillary electrochromatography , capillary action , resolution (logic) , aqueous solution , buffer solution , glycopeptide antibiotic , antibiotics , bacteria , materials science , organic chemistry , biochemistry , vancomycin , artificial intelligence , biology , genetics , staphylococcus aureus , computer science , composite material
A new glycopeptide antibiotic, MDL 63,246 (Hepta‐tyr), of the teicoplanin family, has been evaluated in capillary electrophoresis for the resolution of chiral compounds of pharmaceutical and environmental interest. Electrophoretic separations were carried out in a polyacrylamide‐coated capillary using the partial filling‐counter current mode with aqueous‐organic buffers in the pH range 4–6. Experimental parameters affecting resolution, such as antibiotic concentration, buffer pH, organic modifier type and capillary temperature, were studied. The Hepta‐tyr antibiotic exhibited a high enantiorecognition capability towards the studied compounds at very low concentrations (1–2 mg/mL). The optimum experimental conditions were achieved by using a buffer at pH 5 containing acetonitrile at 25°C.