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High resolution capillary electrophoretic separation of oligonucleotides in low‐viscosity, hydrophobically endcapped polyethylene oxide with cubic order
Author(s) -
Magnusdottir Soffia,
Viovy JeanLouis,
François Jeanne
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191029
Subject(s) - dispersity , polymer , dodecane , ethylene oxide , materials science , chemical engineering , capillary electrophoresis , micelle , viscosity , polymer chemistry , copolymer , chemistry , chromatography , organic chemistry , aqueous solution , composite material , engineering
Abstract A triblock self‐associating polymer with the structure n ‐ dodecane‐poly(ethylene oxide)‐ n ‐dodecane and a very low polydispersity has been used as a matrix to separate a sample of single‐stranded oligonucleotides containing Pd(A) 25–30 and Pd(A) 40–60 . Above a concentration of 4%, this associative polymer forms a micellar network with cubic order and a well‐defined micellar spacing, in which the dodecane micellar cores are bridged by polyoxyethylene segments. This medium combines a low viscosity with excellent resolution of oligonucleotides. This work confirms that associative polymers are potentially powerful media for separation in capillary electrophoresis, and argues in favor of the use of monodisperse products presenting a high‐order in the physical gel state.