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On the concept of “normalized buffering power/conductivity ratio” of isoelectric buffers for capillary zone electrophoresis
Author(s) -
Stoyanov Alexander V.,
Righetti Pier Giorgio
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191025
Subject(s) - capillary electrophoresis , conductivity , normalization (sociology) , electrolyte , electrophoresis , analytical chemistry (journal) , linear relationship , capillary action , chemistry , isoelectric focusing , chromatography , range (aeronautics) , materials science , thermodynamics , mathematics , physics , statistics , biochemistry , electrode , sociology , anthropology , composite material , enzyme
The introduction of a new physico‐chemical parameter is proposed: the “normalized buffering power/conductivity ratio”. It expresses the ratio of buffering power to conductivity ( R β/λ ), normalized by the electrolyte concentration, and gives an opportunity to calculate the properties of buffering ions typically used in capillary electrophoretic separations of biopolymers. This procedure of normalization is possible due to the fact that, in the concentration range practivally used, the relationship of R β/λ on concentration is close to linear.