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Immunoblotting of multiple antigenic peptides
Author(s) -
Kurien Biji T.,
Jackson Kenneth,
Scofield R. Hal
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191023
Subject(s) - antigen , computational biology , chemistry , peptide , biology , biochemistry , genetics
Multiple antigenic peptides (MAPs) can be efficiently separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to a nitrocellulose membrane for subsequent use in immunoblot (IgG and IgM). MAPs can be stained by Coomassie and silver on SDS‐PAGE as well as by Fast Green on an immunoblot. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using MAPs. High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands in SDS‐PAGE and immunoblotting.