Premium
Electrophoresis of proteins across a transverse sodium dodecyl sulfate gradient
Author(s) -
Gianazza Elisabetta,
Vignati Mara,
Santi Ombretta,
Vecchio Giuseppe
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191019
Subject(s) - sodium dodecyl sulfate , chemistry , gel electrophoresis , chromatography , electrophoresis , sodium , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , organic chemistry , enzyme
We describe a new protocol for denaturant gradient gel electrophoresis, namely the migration of proteins across transverse sodium dodecyl sulfate (SDS)‐gradients. We show how such gradients may be reproducibly cast, and demonstrate their stability with time once an appropriate SDS reservoir is arranged at the cathode. SDS affects both size and surface charge of the molecules, and influences the secondary and tertiary structure to a variable extent, even in opposite directions. Hence, distinct, sometimes complex, denaturation patterns may be observed for different proteins.