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Theory and practice of isoelectric focusing of interacting systems
Author(s) -
Cann John R.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191010
Subject(s) - isoelectric focusing , isoelectric point , ligand (biochemistry) , chemistry , protein–protein interaction , basis (linear algebra) , chemical physics , computational chemistry , biological system , biochemistry , receptor , biology , mathematics , enzyme , geometry
The theory of mass transport coupled to reversible protein interactions forms the basis for computer simulation of the isoelectric focusing behavior of several model systems. These include pH‐dependent conformational transition, carrier ampholyte‐induced interactions and protein‐ligand interactions. The computational results compare favorably with experimental observations. In addition, a method is formulated for an isoelectric focusing procedure which enables determination of intrinsic ligand‐binding constants for statistical binding of a charged ligand, binding to heterogeneous sites, and cooperative binding.