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Normal and anomalous electrophoretic behavior of polymerase chain reaction‐based DNA polymorphisms in polyacrylamide gels
Author(s) -
Lareu Victoria,
Pestoni Carmela,
Phillips Christopher,
Barros Francisco,
Carracedo Angel,
Court Denise Syndercombe,
Lincoln Patrick
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191007
Subject(s) - amplified fragment length polymorphism , electrophoresis , microsatellite , polymerase chain reaction , typing , dna , polyacrylamide gel electrophoresis , microbiology and biotechnology , polyacrylamide , biology , genetics , chemistry , biochemistry , gene , genetic diversity , allele , enzyme , population , demography , sociology
The electrophoretic mobility of five single tandem repeats (STRs) and four amplified fragment length polymorphisms (AFLPs) in polyacrylamide gels was tested under denaturing and nondenaturing conditions. Relative anomalous mobility in nondenaturing conditions was found in one AT‐rich AFLP (3′ ApoB) and in two AT‐rich STRs (HUMACTBP2 and HUMF13A1). In these cases, highly anomalous electrophoretic behavior was found even when changes were made in the %T value alone. In such cases typing results were affected by the gel composition. The mobility of these systems was nevertheless normal under denaturing conditions. As a consequence of this study, we recommended that the typing of these systems should only be performed under denaturing conditions and we also recommend the further study of the electrophoretic behavior of repetitive DNA polymorphisms, especially AT‐rich systems, before they are used in forensic or genetic applications.