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Quantitative analysis of synthetic dyes in lipstick by micellar electrokinetic capillary chromatography
Author(s) -
Desiderio Claudia,
Marra Carolina,
Fanali Salvatore
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190844
Subject(s) - chromatography , micellar electrokinetic chromatography , detection limit , chemistry , repeatability , tartrazine , analyte , capillary electrophoresis , erythrosine , sodium dodecyl sulfate , lipstick , analytical chemistry (journal) , organic chemistry
The separation of synthetic dyes, used as color additives in cosmetics, by micellar electrokinetic capillary chromatography (MEKC) is described in this study. The separation of seven dyes, namely eosine, erythrosine, cyanosine, rhodamine B, orange II, chromotrope FB and tartrazine has been achieved in about 3 min in an untreated fused silica capillary containing as background electrolyte a 25 m M tetraborate/phosphate buffer, pH 8.0, and 30 m M sodium dodecyl sulfate. The electrophoretic method exhibits precision and relatively high sensitivity. A detection limit (LOD, signal/noise = 3) in the range of 5–7.5 × 10 −7 M of standard compounds was recorded. Intra‐day repeatability of all the studied dye determinations (8 runs) gave the following results (limit values), % standard deviation: 0.24–1.54% for migration time, 0.99–1.24% for corrected peak areas, 0.99–1.24% for corrected peak area ratio (analyte/internal standard) and 1.56–2.74% for peak areas. The optimized method was successfully applied to the analysis of a lipstick sample where eosine and cyanosine were present.