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The combination of Indian ink staining with immunochemiluminescence detection allows precise identification of antigens on blots: Application to the study of glycosylated barley storage proteins
Author(s) -
Eynard Lucia,
Laurière Michel
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190833
Subject(s) - blot , staining , chemiluminescence , glycoprotein , microbiology and biotechnology , chemistry , chromatography , biology , biochemistry , genetics , gene
The localization after blotting of specific spots in two‐dimensional electrophoretic protein pattern was achieved using, in that order, Indian ink protein staining and immunodetection with chemiluminescence on the same membrane. Indian ink did not inhibit significantly the antibody reactions even after overnight staining. It produces permanent staining that did not quench the chemiluminescent signal, recorded on a film. This allowed perfect matching between the specific and the total protein patterns. The procedure was applied to the identification of glycoproteins present in barley storage protein preparations.