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A 33 bp minisatellite repeat upstream of the ‘mutated in colon cancer’ gene at chromosome 5q21
Author(s) -
Bugert Peter,
Kenck Christiane,
Kovacs Gyula
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190827
Subject(s) - minisatellite , genetics , locus (genetics) , biology , loss of heterozygosity , gene , microbiology and biotechnology , allele , chromosome , southern blot , microsatellite
We have found a 33 bp minisatellite repeat in the 5′‐flanking region of the mutated in colon cancer (MCC) gene at chromosome 5q21. Southern blot experiments demonstrated the locus specificity of the repeat. The number of repeat units varied between 5 and 11 with a heterozygosity of 0.56. The sequence 5′‐AGG AGT GTG AAT GGG GCA TAG TGA ATG AGG GGA‐3′ of the repeat units does not match the consensus sequence of chi‐related minisatellites. The minisatellite is not expressed as part of a gene transcription unit. However, it can be used as a tool for the detection of allelic changes at chromosome 5q21 on standard agarose gels.