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Amplification of microsatellites adapted from human systems in faecal DNA of wild Hanuman langurs ( Presbytis entellus )
Author(s) -
Launhardt Kristin,
Epplen Cornelia,
Epplen Jörg T.,
Winkler Paul
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190826
Subject(s) - biology , microsatellite , zoology , dna , genetics , gene , allele
Microsatellite systems originally established for human DNA were utilized for paternity testing from faecal DNA in a natural population of Hanuman langurs ( Presbytis entellus ). Thirty‐two primer pairs were applied to amplify DNA obtained from langur faeces. Twenty‐two of these primer pairs yielded specific amplification products and 11 loci were polymorphic. Allele distributions and heterozygosity rates were determined for five systems. Genetic information from these five systems was sufficient for paternity exclusion in 46 out of 52 cases. Results were consistent enough to allow genotyping from faeces, although sometimes only one allele was amplified in a heterozygous individual. In conclusion, relationship analyses from faeces are possible in spite of the evolutionary distance between humans and langurs.