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Incorporation of dispersion into the theory of electrophoresis of DNA and its complexes
Author(s) -
Cann John R.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190822
Subject(s) - electrophoresis , dispersion (optics) , dna , macromolecule , phase (matter) , gel electrophoresis , biophysics , chemistry , materials science , chemical physics , chromatography , optics , physics , biology , biochemistry , organic chemistry
Incorporation of the dispersion coefficient into the theory of the mobility‐shift assay for DNA‐protein complexes was highly successful largely due to increased mathematical rigor. A model simulating electrophoretic migration of DNA across the phase boundary between the initial zone of macromolecule and the gel lane predicts the peak asymmetry observed experimentally. It also predicts that, under the agency of the dispersion coefficient, the peak will become progressively more symmetrical during migration along the gel lane.