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Correlation of plasma protein separation patterns obtained by two‐dimensional polyacrylamide gel electrophoresis and by capillary electrophoresis
Author(s) -
Manabe Takashi,
Miyamoto Hiromitsu,
Oota Hideto,
Toyota Yosuke
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190819
Subject(s) - chromatography , capillary electrophoresis , gel electrophoresis of proteins , electrophoresis , free flow electrophoresis , chemistry , polyacrylamide gel electrophoresis , separation (statistics) , gel electrophoresis , biochemistry , computer science , machine learning , enzyme
We used three different electrophoretic techniques for the analysis of human plasma proteins: (i) two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE), with sodium dodecyl sulfate (SDS) used only in slab gel electrophoresis; (ii) capillary isoelectric focusing (CIEF) with no denaturants; (iii) linear polyacrylamide (LPA)‐filled capillary electrophoresis with SDS (SDS‐CE). With technique (i), data on isoelectric point and molecular size of plasma proteins can be obtained. Techniques (ii) and (iii) are suited to obtain quantitative information on proteins. The separation principle used in technique (ii) is closely related to that used in the first dimension of technique (i), and that used in technique (iii) related to that in the second dimension of technique (i). Therefore, we could successfully correlate protein separation patterns obtained by 2‐D PAGE and those obtained by capillary electrophoresis. The advantages of correlating data obtained by various electrophoretic techniques in the course of constructing a comprehensive database on human plasma proteins are discussed.