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Subtyping of transferrin by isoelectric focusing in immobilized pH gradients
Author(s) -
Guo Yaojun,
Li Xinhui,
Zhu Haizhen,
Shen Ziwei
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190817
Subject(s) - isoelectric focusing , transferrin , subtyping , population , allele frequency , chemistry , isoelectric point , microbiology and biotechnology , allele , phenotype , biology , gene , biochemistry , medicine , enzyme , environmental health , computer science , programming language
Isoelectric focusing in immobilized pH gradients ranging from pH 5.05 to 5.60 was used to study the distribution of transferrin (Tf) subtypes and their gene frequencies from 188 unrelated healthy donors of the Han population in Beijing. Six phenotypes (TfCl, TfC2, TfC1C2, TfC1C3, TfC1D chi and TfC2D chi ) were detected and Tf*C3 has never before been reported in the Han population. Gene frequencies were as follows: Tf*Cl = 0.7420, Tf*C2 = 0.2420, Tf*C3 = 0.0027, Tf*Dchi = 0.0133. There is good agreement between the observed and expected values corresponding to the Hardy‐Weinberg equilibrium (∑χ 2 = 0.9183, df = 2, 0.5 < P , < 0.75). The allele frequencies for Tf*C1, Tf*C2 and Tf*Dchi agreed with those previously reported for the Chinese Han population.