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Continuous flow isoelectric focusing for purification of proteins
Author(s) -
Soulet Nathalie,
Rouxde Balmann Hélène,
Sanchez Victor
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190813
Subject(s) - isoelectric focusing , bovine serum albumin , chromatography , isoelectric point , chemistry , albumin , bovine milk , serum albumin , dispersion (optics) , biochemistry , physics , optics , enzyme
To estimate the feasibility of isoelectric focusing in a continuous mode, the conditions for obtaining stable and linear pH gradients were first investigated, using a mixture of carrier ampholytes. Then the operating parameters allowing the isoelectric focusing of bovine serum albumin as model protein were determined. A theoretical approach was used to find conditions minimizing the electrohydrodynamic effect, mainly responsible for protein dispersion, for the separation of bovine serum albumin and α‐lactalbumin from bovine milk.