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Electrophoretic analysis of multiple protein‐DNA interactions
Author(s) -
Fried Michael G.,
Daugherty Margaret A.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190804
Subject(s) - electrophoresis , dna , macromolecule , resolution (logic) , stoichiometry , chemistry , high resolution , chemical physics , biophysics , computational biology , biology , chromatography , biochemistry , computer science , remote sensing , artificial intelligence , geology
Under favorable conditions, native gel electrophoresis allows the resolution of protein‐DNA complexes that differ in stoichiometry, identities of occupied DNA sequences (configuration), and macromolecular conformation. This technique provides a unique opportunity to analyze, in thermodynamic terms, the molecular interactions that govern the equilibrium distributions of species in protein‐DNA mixtures. Here we describe a general theoretical approach to the analysis of electrophoretic band intensities, and provide examples of its application to the analysis of several interacting systems.