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Application of free‐flow electrophoresis for isolation and purification of proteins and peptides
Author(s) -
Loseva Olga I.,
Gavryushkin Alexander V.,
Osipov Vladimir V.,
Vanyakin Evgeny N.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190712
Subject(s) - free flow electrophoresis , isolation (microbiology) , chromatography , electrophoresis , chemistry , gel electrophoresis of proteins , gel electrophoresis , biochemistry , polyacrylamide gel electrophoresis , biology , microbiology and biotechnology , enzyme
Free‐flow electrophoresis (FFE) has been applied to the separation and purification of a variety of proteins and polypeptides: bee venom, tumor necrosis factor, interleukin‐1β, interferon‐α and superoxide dismutase. FFE at constant pH and conductivity of the carrying buffer is shown to be efficient at various separation schemes. In some cases, the method allows us to obtain proteins with a purity of more than 90% at a productivity of 20–30 mg/h. An electrophoretic apparatus with a new, multi‐sectional construction of the electrophoretic chamber and a system for cross‐displacement of carrying buffer in the chamber is described.