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Automated free‐solution isotachophoresis: Instrumentation and fractionation of human serum proteins
Author(s) -
Böttcher Alfred,
Möllers Christoph,
Lackner Karl J.,
Schmitz Gerd
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190710
Subject(s) - isotachophoresis , chromatography , fractionation , chemistry , agarose , electrophoresis , electrolyte , capillary electrophoresis , capillary action , instrumentation (computer programming) , analytical chemistry (journal) , materials science , electrode , computer science , composite material , operating system
An automated free‐solution isotachophoresis system (FS‐ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200‐fold higher sample load compared to separation in capillaries of 180 μm inner diameter as used in analytical capillary isotachophoresis (C‐ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C‐ITP, which is essential for preparative‐scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C‐ITP and agarose gel electrophoresis.