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Two‐dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome
Author(s) -
Rabilloud Thierry,
Kieffer Sylvie,
Procaccio Vincent,
Louwagie Mathilde,
Courchesne Paul L.,
Patterson Scott D.,
Martinez Pascal,
Garin Jerome,
Lunardi Joël
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190616
Subject(s) - proteome , proteomics , mitochondrion , computational biology , biology , organelle , human proteome project , mass spectrometry , compartmentalization (fire protection) , human placenta , identification (biology) , microbiology and biotechnology , bioinformatics , biochemistry , chemistry , genetics , placenta , gene , chromatography , enzyme , botany , pregnancy , fetus
Owing to the complexity of higher eukaryotic cells, characterization of a complete proteome is likely to be difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized tools to study specifically the biology and "physiology" of the target organelle. Within this frame, we report here the construction of the human mitochondrial proteome, using placenta as the source tissue. Protein identification was carried out mainly by peptide mass fingerprinting, but other methods were also used (N-terminal microsequencing, blotting). The optimization steps in two-dimensional (2-D) electrophoresis needed for proteome research are discussed. However, the relative paucity of data concerning mitochondrial proteins is still the major limiting factor in building the corresponding proteome, which should be a useful tool for researchers working on human mitochondria and their deficiencies.