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Alterations in the expression of P 2X1 receptors in failing and nondiseased human atria
Author(s) -
Berry Desiree,
Yao Mu,
Barden Julian A.,
Balcar Vladimir J.,
Hansen Mitchell A.,
Bennett Maxwell R.,
Keogh Anne,
dos Remedios Cristobal G.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190542
Subject(s) - dilated cardiomyopathy , blot , receptor , cardiomyopathy , human heart , biology , medicine , protein subunit , endocrinology , cardiology , pathology , heart failure , biochemistry , gene
This is the first report of the analysis of the ATP‐specific P 2X1 receptor subunit in human hearts. We have examined homogenate samples of human left atria for the presence of P 2X1 receptors using Western blots. Anti‐P 2X1 immunoreactivity was detected in populations of nondiseased atria as well as in atria from explanted hearts from patients with terminally failing heart conditions such as dilated cardiomyopathy. At least three groups of P 2X1 immunoreactive proteins were detected in the Western blots with approximate molecular mass values of 50, 70, and 160 kDa. We report changes in expression of their 50 and 70 kDa components. These changes may be related to the type of deficit in these hearts since the changes have been observed in hearts with decreased ejection fractions characteristic of dilated cardiomyopathy.