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Development of mini‐gel technology in two‐dimensional electrophoresis for mass‐screening of samples: Application to tears
Author(s) -
Glasson Melissa J.,
Molloy Mark P.,
Walsh Bradley J.,
Willcox Mark D. P.,
Morris Carol A.,
Williams Keith L.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190541
Subject(s) - chromatography , tears , gel electrophoresis , electrophoresis , chemistry , polyacrylamide gel electrophoresis , contact lens , capillary electrophoresis , artificial tears , ophthalmology , medicine , surgery , biochemistry , enzyme
Despite the extensive literature available on tear proteins and lipids, very little has been reported on the tear fluid as a whole and it's changes in contact lens wear or ocular diseased patients. Initially a human reflex tear two‐dimensional map was created by Molloy et al. ( Electrophoresis 1997, 18 , 2811–2815), using this information a process for mass‐screeniung was established. The large format two‐dimensional technique was evaluated, using a basal tear reference map, and modified to describe a fast, efficient and cost effective method of protein separation. The use of one pH 3–10 18 cm nonlinear immobilised pH gradient (IPG) strip and two mini‐gels for the second‐dimensional sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) results in an effective separation of tear proteins which will be applied in diagnostic studies of tear samples.