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Development of a two‐dimensional gel electrophoresis database of human lysosomal proteins
Author(s) -
Chataway Timothy K.,
Whittle Alison M.,
Lewis Martin D.,
Bindloss Colleen A.,
Moritz Robert L.,
Simpson Richard J.,
Hopwood John J.,
Meikle Peter J.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190538
Subject(s) - gel electrophoresis , organelle , two dimensional gel electrophoresis , electrophoresis , cell , database , cell type , biology , chemistry , computational biology , biochemistry , microbiology and biotechnology , proteomics , computer science , gene
Abstract Two‐dimensional gel electrophoresis databases have been generated for a range of tissue cell and fluid types, from a number of species. A major difficulty in the development of such databases is the large number of proteins present in even a single cell type (> 10 000) and the low levels of many of these proteins. One approach to overcome these difficulties is to fractionate the cell into its organelles and generate individual databases for each subcellular component. This has the added advantage of assigning a cellular location to each protein identified. Here we report the development of a two‐dimensional gel electrophoresis database of lysosomal proteins.