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Use of thiourea to increase the solubility of membrane proteins in two‐dimensional electrophoresis
Author(s) -
Rabilloud Thierry
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190526
Subject(s) - thiourea , solubility , electrophoresis , membrane protein , chemistry , chromatography , membrane , biochemistry , organic chemistry
The separation of membrane proteins by high‐resolution two‐dimensional electrophoresis was carried out. At high loads, these proteins are prone to precipitation, resulting in poor resolution. It is shown here that the use of thiourea, previously described for focusing in immobilized pH gradients, can be extended to conventional isoelectric focusing. As thiourea inhibits acrylamide polymerization, a modified photopolymerization system must be used. These modifications result in higher solubility of proteins during IEF, thereby increasing the resolution and capacity of the two‐dimensional gels.