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Enhanced separation of antidepressant drugs using a polymerized nonionic surfactant as a transient capillary coating
Author(s) -
Harrell Crystal W.,
Dey Joykrishna,
Shamsi Shahab A.,
Foley Joe P.,
Warner Isiah M.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190519
Subject(s) - nortriptyline , desipramine , micellar electrokinetic chromatography , chromatography , chemistry , capillary electrophoresis , doxepin , micelle , amitriptyline , antidepressant , aqueous solution , organic chemistry , pharmacology , neuroscience , biology , hippocampus , medicine
The separation of seven structurally similar antidepressant drugs (amitriptyline, nortriptyline, imipramine, desipramine, protriptyline, doxepin, and nordoxepin) was achieved in under 15 min using a novel nonionic micelle polymer, poly( n ‐undecyl‐α‐ D ‐glucopyranoside) (PUG) by use of capillary zone electrophoresis (CZE). Systematic studies with varying polymer concentration, pH, and percent organic modifier were conducted in order to find the optimum conditions for baseline separation of the seven tricyclic antidepressants. In addition, equations for capacity factor were used to estimate the extent of what was initially thought to be micelle analyte interaction. A series of calculations show that a modified CZE system (PUG‐CZE) was the actual mode of separation. Thus, our study concluded that PUG functioned in a non‐electrokinetic chromatography mode.