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Simultaneous genotyping of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci by amplified product length polymorphism (APLP) analysis
Author(s) -
Aoshima Toshiaki,
Umetsu Kazuo,
Yuasa Isao,
Watanabe Gotaro,
Suzuki Tsuneo
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190508
Subject(s) - aldh2 , genotyping , alcohol dehydrogenase , aldehyde dehydrogenase , biology , genetics , polymerase chain reaction , genotype , allele , polymorphism (computer science) , adh1b , microbiology and biotechnology , alcohol , enzyme , dehydrogenase , biochemistry , gene , branched chain alpha keto acid dehydrogenase complex
Genotyping of the alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci is important in alcohol studies. We describe a method for simulataneous genotyping of ADH2 and ALDH2 based on amplified product length polymorphism (APLP) analysis. Two polymerase chain reaction (PCR) fragments for ADH2 (57 bp, 53 bp) and two for ALDH2 (78 bp, 73 bp) are simultaneously amplified. Nine banding patterns reflecting the genotypes of the ADH2 and ALDH2 loci are clearly and unambiguously distinguished. The APLP method seems to be particularly suited for large‐scale population studies of ADH2 and ALDH2 loci because it is simple and rapid.