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Enhanced hybridization labeling signals in Southern blotted DNAs fractionated with voltage gradient gel electrophoresis
Author(s) -
Barbieri Rainer,
Duro Giovanni,
Izzo Vincenzo
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190505
Subject(s) - gel electrophoresis , nucleic acid , electrophoresis , temperature gradient gel electrophoresis , chemistry , dna , peptide nucleic acid , chromatography , microbiology and biotechnology , biology , biochemistry , gene , 16s ribosomal rna
An enhancement of hybridization labeling signals is demonstrated in Southern blotted DNAs, fractionated by voltage gradient gel electrophoresis. This enhancement is due to a reduced thickness of each single nucleic acid band in the gel as a consequence of the gradient effect, corresponding to an increased concentration of DNA per unit area.