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High‐throughput robotic system for sequencing of microbial genomes
Author(s) -
Hilbert Helmut,
Schäfer Andreas,
Collasius Michael,
Düsterhöft Andreas
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190408
Subject(s) - shotgun sequencing , dna sequencing , plasmid , computational biology , dna nanoball sequencing , shotgun , dna , massive parallel sequencing , genome , biology , genomic library , genetics , gene , base sequence
A high‐throughput robotic workstation system was used for double‐stranded plasmid DNA template preparation and sequencing reaction setup to streamline the sequencing process in genome projects. All 96‐well miniprep kits that were tested provided high quality plasmid DNA suitable for fluorescent DNA sequencing. After quantitation in a 96‐well UV spectrophotometer, the plasmid DNA was used as template to automatically set up sequencing reactions. The setup was controlled by spread sheets that were imported into the robotic system. We utilized this integrated system to prepare all necessary shotgun templates for our contributions to a number of large‐scale genome projects as well as a full‐length cDNA sequencing project.