Premium
Hybridization mapping of Trypanosoma cruzi chromosomes III and IV
Author(s) -
Hanke Jens,
Frohme Marcus,
Laurent JeanPierre,
Swindle John,
Hoheisel Jörg D.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190405
Subject(s) - cosmid , biology , genetics , chromosome , trypanosoma cruzi , clone (java method) , genome , genomic library , bacterial artificial chromosome , gene mapping , microbiology and biotechnology , dna , gene , parasite hosting , base sequence , world wide web , computer science
As part of the Trypanosoma Genome Initiative launched by the World Health Organization (WHO), a physical clone map of Trypanosoma cruzi chromosomes III and IV was generated to facilitate both DNA sequence analysis of the parasite's genome and the investigation of chromosome organization. Apart from a few genetic markers, anonymous cosmids were taken from chromosomal sublibraries and individually hybridized to filter arrays of the relevant cosmid library. The probe order was determined from the hybridization fingerprint results and used to define a fitting clone order, with few gaps remaining. The results were independently verified by hybridizations to a bacterial artificial chromosome (BAC) library and, in case of chromosome III, restriction mapping. For gap closure, additional experiments on a total cosmid library were carried out. The possible tiling paths consist of 26 clones for chromosome III (610 kbp) and 28 clones for chromosome IV (680 kbp).