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Base‐specific separation of oligodeoxynucleotides by capillary affinity gel electrophoresis
Author(s) -
Baba Yoshinobu,
Sawa Tomohiro,
Kishida Akio,
Akashi Mitsuru
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190312
Subject(s) - capillary electrophoresis , chromatography , chemistry , free flow electrophoresis , electrophoresis , separation (statistics) , gel electrophoresis , separation method , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , computer science , biochemistry , enzyme , machine learning
Capillary affinity gel electrophoresis was applied to sequence‐specific and base composition‐specific recognition of oligodeoxynucleotides, utilizing the formation of heteroduplexes between a nucleic acid analogue immobilized into the capillary gel and soluble oligodeoxynucleotides with different sequences. Capillary affinity gel electrophoresis using capillaries filled with a conjugated gel of polyacrylamide and a synthetic nucleic acid analogue [poly(9‐vinyladenine)] was effective for the selective separation of hexathymidylic acid from a mixture of four homopolymers of A 6 , C 6 , G 6 , and T 6 and for the complete resolution of five heteropolymers of hexadeoxynucleotides (T, TTAAAA, TTTAAA, TTTTAA, A). We also demonstrated that capillary affinity gel electrophoresis was useful for the selective and the sensitive sequence‐specific recognition of sequence isomers of DNA (TTTTAA, TTTTAAT, TTTATA, and TTTTAA).