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Viscosity‐adjustable block copolymer for DNA separation by capillary electrophoresis
Author(s) -
Wu Chunhung,
Liu Tianbo,
Chu Benjamin
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190216
Subject(s) - capillary electrophoresis , copolymer , electrophoresis , materials science , capillary action , ethylene oxide , polymer , propylene oxide , chromatography , viscosity , polyacrylamide , chemical engineering , chemistry , polymer chemistry , composite material , engineering
The viscosity‐adjustable property of F127 block copolymer PEO 99 PPO 69 PEO 99 , PEO and PPO being poly (ethylene oxide) and poly(propylene oxide), respectively, was found to be useful for the development of automated capillary electrophoresis (CE). The polymer solution can form a gel‐like structure with sieving ability and can also serve as a dynamic coating material, thereby effectively suppressing the electroosmotic flow induced by the ionization of the silanol group on the quartz capillary inner wall. When applied to CE as a separation medium, F127 block copolymer can provide the advantages of high separation resolution, easy injection and replacement of the triblock copolymer solution and convenient capillary column treatment. High reproducibility of DNA electrophoretic migration time in CE by replenishing F127 solution in acid‐washed capillary tubings can be achieved. The relative standard deviation of the DNA migration time is less than 2%. In the investigation of F127 concentration and temperature effects on the performance of DNA separation in CE, we have found that the DNA electrophoretic migration behavior in the F127 gel‐like solution cannot be described by any one of the existing models.