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Oligonucleotide DNA fingerprinting optimized to determine parentage in three beetle species
Author(s) -
Benken Theodor,
Müller Josef K.,
Peschke Klaus
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190205
Subject(s) - biology , dna profiling , locus (genetics) , zoology , genetics , evolutionary biology , dna , gene
Oligonucleotide fingerprinting was performed on three beetle species from different families (Coleoptera: Staphylinidae: Aleochara curtula ; Silphidae: Nicrophorus vespilloides ; Tenebrionidae: Blaps lethifera ) to obtain detailed information on parentage in mating systems. We report variations in the number of hybridizing fragments for the different species depending on the combinations of probes and restriction enzymes used. In addition to conventional multilocus fingerprint patterns, we established a single locus system for A. curtula (GTG 5 ; Hin fI) and described an oligolocus system in N. vespilloides (GATA 4 ; Hae III).