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Nonradioactive sequence‐tagged microsatellite site analyses: A method transferable to the tropics
Author(s) -
Lagoda Pierre J. L.,
Dambier Dominique,
Grapin Agnès,
Baurens FrancChristophe,
Lanaud Claire,
Noyer JeanLouis
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190204
Subject(s) - microsatellite , tropics , sequence (biology) , environmental science , biology , ecology , genetics , allele , gene
Utilization of existing isozyme analysis facilities to detect sequence‐tagged microsatellite site (STMS) polymorphism or any simple sequence repeat (SSR) variation is described. Different parameters concerning the difficulties in transferring molecular techniques to less sophisticated laboratory infrastructures ( i. e. tropical outstations) are discussed ( e.g. reproducibility, efficacy, precision). Nonradioactive STMS analysis is bound to foster collaborative research between “biodiversity” and “biotechnology” centers.