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DNA fingerprinting of Listeria monocytogenes using enterobacterial repetitive intergenic consensus (ERIC) motifs‐polymerase chain reaction/capillary electrophoresis
Author(s) -
Sciacchitano Carl J.
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190112
Subject(s) - dna profiling , genomic dna , biology , listeria monocytogenes , capillary electrophoresis , polymerase chain reaction , agarose gel electrophoresis , intergenic region , listeria , dna , gel electrophoresis , microbiology and biotechnology , genetics , bacteria , genome , gene
The molecular technique, enterobacterial repetitive intergenic consensus (ERIC)‐polymerase chain reaction (PCR) produces genomic DNA fingerprint that discriminate bacterial species and strains. This technique was applied to the characterization of Listeria monocytogenes , an important food‐borne pathogen implicated in numerous cases of listeriosis. The ERIC‐PCR resulted in distinct DNA fingerprinting patterns of all L. monocytogene serotypes and Listeria species. Analysis of the genomic DNA fingerprints was accomplished using capillary electrophoresis (CE), an alternative technique to the conventional agarose gel method. The optimization of CE conditions (electrokinetic injection, applied voltage) resulted in the resolution of amplified DNA fragments up to 1000 bp. Comparisons of electropherograms provided genomic fingerprint templates which could be further used for supplementary information. The ERIC‐PCR method coupled to CE provides a rapid technique in differentiating bacterial spp., and may contribute relevant information in food‐borne outbreak studies.