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Strategies for the capillary electrophoretic separation of indole alkaloids in Psilocybe semilanceata
Author(s) -
PedersenBjergaard Stig,
Rasmussen Knut Einar,
Sannes Ellen
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150190107
Subject(s) - capillary electrophoresis , micellar electrokinetic chromatography , chemistry , derivatization , chromatography , electrophoresis , indole test , cationic polymerization , electrokinetic phenomena , capillary electrochromatography , micelle , organic chemistry , high performance liquid chromatography , aqueous solution
While the hallucinogenic mushrooms Psilocybe semilanceata have previously been analyzed for the indole alkaloids psilocybin and baeocystin by capillary zone electrophoresis (CZE) at pH 11.5, the present work focused on the development of an alternative and complementary capillary electrophoretic method for their identification. Owing to their structural similarity and zwitterionic nature, the compounds were difficult to resolve based on different interactions with cationic or anionic micelles. However, while the attempts with micellar electrokinetic chromatography (MEKC) were unsuccessful, rapid derivatization with propyl chloroformate and reanalysis by CZE at pH 11.5 was effective to support identification of the two indole alkaloids. Psilocin was difficult to analyze by CZE at pH 11.5 owing to comigration with the electroosmotic flow. For this compound, the pH of the running buffer was reduced to 7.2 to effectively enhance the electrophoretic mobility.